Tissue Factor Pathway Inhibitor [Tfpi]: A Natural Coagulation Inhibitor and Potential Therapeutic Agent – A Review

Recent Progress on Tissue Factor Pathway Inhibitor (TFPI)

外 因 系凝 固 の 開 始 反 応 で あ る組 織 因子 に よ る VII因 子 お よびX因 子 の 活 性 化 を特 異 的 に 阻 害 す る プ ロ テ ア ー ゼ イ ン ヒ ビタ ー の 存 在 は 古 くか ら指 摘 さ れ て きた が,そ の性 質 は長 い 間 明 らか で は な か っ た.そ の後,Brozeら の研 究 に よ り, そ の イ ン ヒ ビタ ー はXa因 子 依 存 性 にVII因 子 の 活 性 化 を阻 害 す る作 用 を もつ とい う一 見,極 め て 奇 妙 な イ ン ヒ ビタ ー で あ る こ とが 明 らか に な っ て きた.1988年 に彼 らに よ り,そ の ア ミノ 酸 配 列 が 決 定 さ れ,そ れ 以 後,い くつ か の グル ー プ に よ り ,そ の組 換 え体 が 調 製 され た ...

A novel anticoagulant activity assay of tissue factor pathway inhibitor I (TFPI).

Tissue factor (TF) pathway inhibitor I (TFPI) is the physiological inhibitor of TF-induced blood coagulation. Circulating blood contains full-length TFPI and TFPI truncated at the C-terminal end. Previous studies have shown that full-length TFPI exerts a stronger anticoagulant effect on diluted prothrombin time (DPT) than truncated TFPI, and it has been suggested that full-length TFPI is biolog...

Matrix metalloproteinases cleave tissue factor pathway inhibitor. Effects on coagulation.

The capacity of inflammatory cell-derived matrix metalloproteinases (MMPs) to cleave tissue factor pathway inhibitor (TFPI) and alter its activity was investigated. MMP-7 (matrilysin) rapidly cleaved TFPI to a major 35-kDa product. In contrast, MMP-1 (collagenase-1), MMP-9 (gelatinase B), and MMP-12 (macrophage elastase) cleaved TFPI into several fragments including the 35-kDa band. However, ra...

Coagulation Therapy in Hemophilia A and its Relation to Factor VIII Inhibitor in Northeast of Iran

Cloning, expression, and characterization of mouse tissue factor pathway inhibitor (TFPI).

Tissue factor pathway inhibitor (TFPI) acts to regulate the initiation of coagulation by first inhibiting factor Xa. The complex of factor Xa/TFPI then inhibits the factor VIIa/tissue factor complex. The cDNA sequences of TFPI from several different species have been previously reported. A high level of similarity is present among TFPIs at the molecular level (DNA and protein sequences) as well...